Clinical Trial: Safety of Stem Cells Intrabronchial Instillation for Silicosis

Study Status: Completed
Recruit Status: Completed
Study Type: Interventional

Official Title: Phase-1 Study of Autologous Bone Marrow Cells Intrabronchial Instillation for Patients Silicosis

Brief Summary: The aim of this study was to analyze the safety, pulmonary function, and quality of life data of patients with silicosis treated with intrabronchial instillation of bone marrow derived mononuclear cells (BMDMC, 2x107) through bronchoscopy.

Detailed Summary:

This study will perfume the safety (Phase I) study of 10 patients with silicosis treated with intrabronchial instillation of autologous bone marrow derived mononuclear cells (BMDMC, 2x107) through bronchoscopy. The inclusion criteria is: age between 18-50, chronic and accelerated silicosis, characterized with a fibrotic increase in the last two years, FEV1 <60% and > 40%, FVC > 60% and SaO2 >90%, while the exclusion criteria were: smoking, active tuberculosis or other infections, cancer, auto-immune disorders, hematological, hepatic or cardiac diseases, and pregnancy. All patients will be subjected to clinical examination, answered questionnaires of quality of life (SGRQ and SF36) and dyspnea score (Borg), performed high resolution CT of thorax, pulmonary function tests with DLCO and 6-minute walk test and lung perfusion scintigraphy before and 7, 30, 60, 180 and 360 days after treatment.

For each patient, 2×107 cells will be labeled with 99mTc. Briefly, 500 μl of sterile SnCl2 solution is added to the cells and the mixture is incubated at room temperature for 10 min. Forty-five mCi of 99mTc is then added and incubation continued for another 10 min. After centrifugation (500×g for 5 min), the supernatant is removed and the cells are washed in saline solution. The pellet was also resuspended in saline solution. Viability of the labeled cells was assessed by the trypan blue exclusion test, and was estimated to be greater than 93% in all cases. Labeling efficiency (%) was calculated by the activity in the pellet divided by the sum of the radioactivity in the pellet plus supernatant and was estimated to be greater than 90% in all cases. Whole body and planar scans will be performed 2 and 24h after instillation. Perfusion scintigraphy will be performed before and 30, 60, 120 and 180 days after BMDMC therapy. For regional analysis the right and left l
Sponsor: Universidade Federal do Rio de Janeiro

Current Primary Outcome: Absence of lung deficits during the procedure and/or in the 4 months follow-up [ Time Frame: 4 months ]

Original Primary Outcome: Same as current

Current Secondary Outcome: Improvement of pulmonary deficits [ Time Frame: 1 year ]

Original Secondary Outcome: Same as current

Information By: Universidade Federal do Rio de Janeiro

Dates:
Date Received: November 10, 2010
Date Started: August 2009
Date Completion:
Last Updated: May 26, 2015
Last Verified: May 2015