Clinical Trial: Ghrelin Resistance in Adolescents With Idiopathic Scoliosis

Study Status: Completed
Recruit Status: Completed
Study Type: Interventional

Official Title: Ghrelin Cellular Resistance Study in Adolescents With Idiopathic Scoliosis

Brief Summary:

Adolescent idiopathic Scoliosis (AIS) is the most common spine pathology. It is opposed to secondary scoliosis due to chronic diseases. Many hypotheses have been made to elucidate the origin of this illness. Recently, the melatonin pathway has been investigated as pinealectomy of the chicken creates a scoliosis that resembles AIS and melatonin supplementation reverses the process. In addition administration of melatonin to AIS patients improved the pathology. However this hypothesis has shown controversial results. Recent studies have demonstrated melatonin cellular resistance in osteoblastic cells from AIS patients. Melatonin acts through G protein coupled receptor (GPCR), mainly using the Gi pathway. In AIS osteoblasts, this pathway is blocked leading to a decrease in the inactivation of the adenylyl cyclase and therefore maintenance of high level of cyclic adenosine monophosphate (cAMP) concentrations in the cells. As modulation of cAMP is important for osteogenesis such resistance may be critical for the initiation or the development of AIS.

Gi signalization is used by several other GPCR, thus, this hormonal resistance could logically be found in other hormonal or mediator pathways. A precedent study previously focused on ghrelin in AIS, and demonstrated that AIS patients possess elevated plasmatic values of ghrelin. This study also observed decreased response to ghrelin in AIS cultures osteoblasts.


Detailed Summary:
Sponsor: University Hospital, Toulouse

Current Primary Outcome: Testing ghrelin cellular resistance by intracellular cAMP dosage [ Time Frame: baseline ]

Testing ghrelin cellular resistance in AIS by measuring the variation of the intracellular cAMP level in response to ghrelin in osteoblast cells in vitro of AIS patients versus controls


Original Primary Outcome: Same as current

Current Secondary Outcome:

  • ghrelin level in serum [ Time Frame: baseline ]
    Measure the ghrelin level in the serum of AIS patients versus controls
  • ghrelin levels in cellular supernatants [ Time Frame: baseline ]
    Measure the ghrelin levels in cellular supernatants of osteoblasts from AIS and controls patients in order to evaluate the ghrelin production of the cells.


Original Secondary Outcome: Same as current

Information By: University Hospital, Toulouse

Dates:
Date Received: July 4, 2016
Date Started: March 2010
Date Completion:
Last Updated: May 10, 2017
Last Verified: May 2017