Clinical Trial: PCR Assay for Diagnosis of Sarcoptes Scabiei

Study Status: Recruiting
Recruit Status: Recruiting
Study Type: Observational

Official Title: PCR Assay for Diagnosis of Sarcoptes Scabiei

Brief Summary: The main objective of this study is to identify unique DNA sequences within the genome of human scabies that can be utilized to identify the parasite through PCR.

Detailed Summary: The main objective of this proposed study is to identify unique DNA sequences within the genome of human scabies that can be utilized to identify the parasite through polymerase chain reaction or PCR. The goal of the project is to design an assay that can distinguish samples from skin scrapings containing scabies mites, eggs, or fecal material confirmed by clinic based microscopic evaluation (gold standard) from negative controls (i.e. scrapings for tinea and/or demodex folliculitis) that do not contain Sarcoptes scabiei. We hypothesize that specifically amplifying the unique regions of the Scabies genome using PCR can serve as a means to diagnosis infestations in humans. To address this question, we will optimize PCR amplification of known scabies samples then apply our procedure to DNA extracted from patient samples.
Sponsor: Seton Healthcare Family

Current Primary Outcome: Identification of DNA sequences within scabies that can be used to identify the parasite through PCR [ Time Frame: 1 year ]

Original Primary Outcome: Same as current

Current Secondary Outcome:

Original Secondary Outcome:

Information By: Seton Healthcare Family

Dates:
Date Received: September 29, 2014
Date Started: October 2014
Date Completion: December 2017
Last Updated: February 14, 2017
Last Verified: February 2017