Clinical Trial: Studying Protein Expression in Tissue Samples From Younger Patients With Rhabdomyosarcoma

Study Status: Completed
Recruit Status: Completed
Study Type: Observational

Official Title: Tissue Microarray Studies of the 12q13-q14 Amplicon in Alveolar Rhabdomyosarcoma

Brief Summary: This research trial studies protein expression in tissue samples from younger patients with rhabdomyosarcoma. Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. It may also help doctors find better ways to treat cancer.

Detailed Summary:

PRIMARY OBJECTIVES:

I. To investigate (in two stages) which proteins are overexpressed in association with 12q13-q14 amplification by applying fluorescence in situ hybridization (FISH) and immunohistochemical (IHC) assays to a panel of alveolar rhabdomyosarcomas (ARMS) cases organized into tissue microarrays.

OUTLINE:

Formalin-fixed paraffin-embedded tissue samples are analyzed for 12q13-q14 frequency and protein overexpression by FISH and IHC.


Sponsor: Children's Oncology Group

Current Primary Outcome: Amplification and fusion status as a result of the 12q13-q14 amplification event [ Time Frame: Baseline ]

Fisher's exact test will be used. For a contingency table other than 2 x 2, the Freeman-Halton test (an extension of Fisher's exact test) will be applied instead.


Original Primary Outcome: Relationship of amplification and fusion status using Fisher's exact test and Freeman-Halton test

Current Secondary Outcome:

Original Secondary Outcome:

Information By: Children's Oncology Group

Dates:
Date Received: May 30, 2012
Date Started: June 2012
Date Completion:
Last Updated: May 16, 2016
Last Verified: May 2016