Clinical Trial: Identification and Characterization of the Methylation Abnormalities on Whole Genome Among Infertile Men

Study Status: Completed
Recruit Status: Completed
Study Type: Observational

Official Title: Identification and Characterization of the Methylation Abnormalities on Whole Genome Among Infertile Men

Brief Summary: This study will analyse the sperm global methylation status of 62 infertile men before assisted reproductive techniques. Some of these patients (20%) present hypomethylation of H19 locus. A global methylation analysis may reveal others imprinting defects.

Detailed Summary:

An increase of the abnormalities of the imprint was brought back at the child's stemming from assisted reproductive techniques. Now abnormalities of methylation could be implied in defects of spermatogenesis and certain abnormalities of development of the male germ cells could be due to modifications abnormal epigenetics.

The objective of this research is to determine the frequency of arisen the abnormalities of methylation at the level of the locus H19 in the sperm cells of barren men presenting an unexplained oligozoospermia and to determine if these changes are a reflection of abnormalities of the profiles of methylation of the whole genome.

The patients will realize a taking of sperm having signed the consent.


Sponsor: Assistance Publique - Hôpitaux de Paris

Current Primary Outcome: Bring to light methylation abnormalities of the locus H19 in men's mature sperm cells presenting an unexplained oligozoospermia [ Time Frame: 1 day ]

Original Primary Outcome: Same as current

Current Secondary Outcome:

  • Determine if these methylation abnormalities of the locus H19 reflect changes in the profile of global methylation of the spermatic DNA [ Time Frame: 1 day ]
  • Estimate the association between these modifications and the nuclear quality of the sperm cell [ Time Frame: 1 day ]
    by TUNEL analysis
  • Estimate the association between these modifications and the rates of success with In VITRO fertilization [ Time Frame: 1 day ]


Original Secondary Outcome: Same as current

Information By: Assistance Publique - Hôpitaux de Paris

Dates:
Date Received: October 22, 2010
Date Started: June 2009
Date Completion:
Last Updated: August 6, 2013
Last Verified: August 2013