Clinical Trial: The Activation of Hippo Pathway in Nasal Polyps

Study Status: Recruiting
Recruit Status: Recruiting
Study Type: Interventional

Official Title: Yap, the Nuclear Target of Hippo Signaling, Regulates Nasal Epithelium Stem Cells Aberrant Proliferation and Differentiation in Nasal Polyps

Brief Summary: Hippo-YAP pathway is a newly discovered pathway controlling the growth, and plays important roles in the regulation of stem cell function and tissue regeneration. Previously we found that a higher expression of YAP in NP, and LPS was able to activate the hippo-YAP pathway in NESCs. Thus, we hypothesize that the aberrant proliferation and differentiation of NESCs could be regulated via the hippo-YAP pathway.

Detailed Summary: Studies have shown that the development of nasal polyps (NP) are closely related to the epithelial remodeling.The investigators have shown that nasal epithelial stem cells(NESCs) derived from NP had reduced growth and proliferation dynamics in vitro culture,and differentiated into ciliated cells with impaired cilia architecture and ciliogenesis.Further studies demonstrated that NESCs were involved in the aberrant damage repair process of the epithelial remodeling in NP.Hippo-YAP pathway is a newly discovered pathway controlling the growth,and plays important roles in the regulation of stem cell function and tissue regeneration.Previously the investigators found that a higher expression of YAP in NP,and LPS was able to activate the hippo-YAP pathway in NESCs.Thus,the investigators hypothesize that the aberrant proliferation and differentiation of NESCs could be regulated via the hippo-YAP pathway,which may result in the epithelial remodeling.In this study,the investigators intend to investigate the mechanism of the hippo-YAP pathway in regulating aberrant proliferation and differentiation of NESCs and explore its upstream signaling and downstream target genes,using the NESCs gas-liquid interface culture system,and a silencing and overexpression of the hippo-YAP pathway component strategy.The investigators aim to unravel the effect and mechanism of the hippo-YAP pathway in the epithelial remodeling of NP,providing new targets and strategy for the improvement or reversal of NP epithelial remodeling.
Sponsor: Third Affiliated Hospital, Sun Yat-Sen University

Current Primary Outcome:

• location of YAP in tissues [ Time Frame: through study completion, an average of 2 years ]
  through Immunohistochemistry
• protein expression level of YAP in tissues [ Time Frame: through study completion, an average of 2 years ]
  through western blot

Original Primary Outcome: Same as current

Current Secondary Outcome:

• expression level of Th1 cytokines in the tissues [ Time Frame: through study completion, an average of 2 years ]
  through Flow cytometry
• expression level of Th17 cytokines in the tissues [ Time Frame: through study completion, an average of 2 years ]
  through Flow cytometry
• activation of hippo-YAP in NSECs on protein aspect [ Time Frame: through study completion, an average of 2 years ]
  Western Blot
• activation of hippo-YAP in NSECs [ Time Frame: through study completion, an average of 2 years ]
  Flow cytometry
• activation of hippo-YAP in NSECs on gene aspect [ Time Frame: through study completion, an average of 2 years ]
  quantitive real time PCR

Original Secondary Outcome: Same as current

Information By: Third Affiliated Hospital, Sun Yat-Sen University

Dates:
Date Received: April 21, 2017
Date Started: April 20, 2017
Date Completion: July 31, 2020
Last Updated: May 15, 2017
Last Verified: May 2017