Clinical Trial: Characterizing the Gut Microbiota Alteration Associated With Severe Acute Malnutrition

Study Status: Completed
Recruit Status: Completed
Study Type: Observational

Official Title: Characterizing the Gut Microbiota Alteration Associated With Severe Acute Malnutrition

Brief Summary: The investigators performed two case-control studies in Niger and Senegal analysing fecal microbiota to characterize the specificity of the gut microbiota alteration associated with severe acute malnutrition (SAM).

Detailed Summary: Fecal samples of cases (severe acute malnutrition (SAM) ascertained according to the WHO definition) and controls (asymptomatic healthy children ascertained by clinical and anthropometric criteria) were analysed by v3v4 16S rRNA sequencing, redox and pH measurement and using specific polymerase chain reaction targeting Methanobrevibacter smithii and Bacteroides thetaiotaomicron. Based on the literature and oxydative stress reported in SAM, the investigators tested if the proportion of aerotolerant prokaryotes is increased among gut prokaryotes enriched in SAM in comparison to prokaryotes enriched in controls. The investigators further linked this to fecal redox potential and assessed the total number of bacteria in feces by flux cytometry.
Sponsor: Institut Hospitalo-Universitaire Méditerranée Infection

Current Primary Outcome: Aerotolerant Odds ratio (AOR) [ Time Frame: 1 day (Participants were not followed as this is a case-contol study only clinical and anthropometric data and fecal samples were collected the day of inclusion) ]

AOR = (number of aerotolerant prokaryotes enriched in the gut of children with SAM * number of obligate anaerobic prokaryotes enriched in controls)/(number of aerotolerant prokaryotes enriched in controls * number of obligate anaerobic prokaryotes enriched in children with SAM)


Original Primary Outcome: Same as current

Current Secondary Outcome:

Original Secondary Outcome:

Information By: Institut Hospitalo-Universitaire Méditerranée Infection

Dates:
Date Received: March 30, 2015
Date Started: January 2014
Date Completion:
Last Updated: April 7, 2015
Last Verified: April 2015