Clinical Trial: Cytokine Profile of the Uterine Secretome After Therapeutic Endometrial Scratching

Study Status: Recruiting
Recruit Status: Recruiting
Study Type: Interventional

Official Title: Cytokine Profile of the Uterine Secretome After Therapeutic Endometrial Scratching

Brief Summary: This study investigates the impact of endometrial scratching on endometrial secretion, when correlated to pregnancy.

Detailed Summary:
Sponsor: University of Aarhus

Current Primary Outcome:

• Cytokine profile of the uterine secretome after therapeutic endometrial scratching [ Time Frame: Within 12 months ]
  The secretome is analyzed using a Multiplex analysis with 45 cytokines, chemokines and growth factors from ProcartaPlex Immunoassay Kit, Affymetrix, eBioscience. The samples are normalized for total protein content using a Nanodrop spectrophometer, and the protein composition is further determined by sodium dodecyl sulphate-polyacrylamide gel electropheresis (SDS-PAGE).
• Immunoassay Kit: [ Time Frame: Within 12 months ]
  BDNF, EGF, Eotaxin, FGF-2, GMCSF, GRO-alpha, HGF, IFN alpha, IFN gamma, IL-1RA, IL-1alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL17-A, IL-18, IL-21, IL-22, IL-23, IL-27, IL-31, IP-10, LIF, MCP-1, MIP-1 alpha, MIP-1 beta, NGF beta, RANTES, PDGF-BB, PÅIGF-1, SCF, SDF-1 alpha, TNF alpha, TNF beta, VEGF-A, VEGF-D

Original Primary Outcome: Same as current

Current Secondary Outcome:

Original Secondary Outcome:

Information By: University of Aarhus

Dates:
Date Received: January 31, 2017
Date Started: October 1, 2016
Date Completion: December 31, 2017
Last Updated: February 1, 2017
Last Verified: October 2016