Clinical Trial: Plasma Triglyceride Lipolysis in Multifactorial Chylomicronemia

Study Status: Completed
Recruit Status: Completed
Study Type: Observational

Official Title: Systematic Study of Post Herapin Lipoprotein Lipase Activity and Lipoprotein Remodelling in Multifactorial Chylomicronemia

Brief Summary: Purpose: The mechanism of most of the multifactorial chylomicronemia (MCM) remains elusive. In order to decipher the mechanisms involved in the occurrence of this disease, plasma TG lipolysis characteristics will be monitored for 60 minutes after heparin injection instead of the 10 minutes gold standard, in a large group of genotyped MCM patients.

Detailed Summary:

Purpose: The mechanism of most of the multifactorial chylomicronemia (MCM) remains elusive. In order to decipher the mechanisms involved in the occurrence of this disease, plasma TG lipolysis characteristics will be monitored for 60 minutes after heparin injection instead of the 10 minutes gold standard, in a large group of genotyped MCM patients.

Method: LPL, APOC2, APOA5, GPIHB1and APOE genotypes will be determined for each patient. Basal lipid profiles including Apo B, CII, CIII, and lipoprotein lipase (LPL) concentration will be measured in 62 MCM patients, in addition to LPL activity (PHLA) T0, T10 T30 T60 minutes, Assessment of TG chylomicron decrease Study of lipoprotein remodelling by agarose gel electrophoresis.

Hypothesis To confirm the preliminary finding of high LPL activity in multifactorial chylomicronemia To explore the interest of a longer assessment of LPL activity following heparin injection. To establish if specific phenotypes could be identified among MCM patient supporting the hypothesis of different mechanisms involved (ie overproduction or defect in hepatic clearance)


Sponsor: Hospices Civils de Lyon

Current Primary Outcome: Lipoprotein Lipase concentration [ Time Frame: 60 minutes after heparin injection ]

Original Primary Outcome: Same as current

Current Secondary Outcome:

  • Basal lipid profiles [ Time Frame: maximum 60 min before heparin injection ]
  • Lipoprotein electrophoresis [ Time Frame: maximum 60 minutes after heparin injection ]
  • Total triglycerides decrease [ Time Frame: maximum 60 minutes after heparin injection ]


Original Secondary Outcome: Same as current

Information By: Hospices Civils de Lyon

Dates:
Date Received: October 12, 2016
Date Started: July 2010
Date Completion:
Last Updated: October 13, 2016
Last Verified: October 2016