Clinical Trial: In Vivo Measurements of Nasal Ciliary Beat Frequency by Using Interferometry

Study Status: Not yet recruiting
Recruit Status: Not yet recruiting
Study Type: Observational

Official Title: In Vivo Measurements of Nasal Ciliary Beat Frequency by Using Interferometry

Brief Summary:

  1. Assessment of a high speed video camera with a green light source for the measurement of ciliary beat frequency (CBF) in the nasal airways of patients.
  2. Assessments of the effect of drugs and other therapies on CBF using the study system.
  3. Comparison of results with standard methods such as ciliary brush biopsies

Detailed Summary:

Background Mucociliary clearance of the airways depends on ciliary movement by ciliated cells that cover the entire airway and the sinuses. In many airway diseases the mucociliary clearance is affected by injury caused by smoke, viruses, other infectious organisms or genetic diseases that are called Primary Ciliary Dyskinesia (PCD).

The diagnosis of PCD is based on a combination of clinical findings such as bronchiectasis, chronic sinusitis, chronic otitis media and immotile sperms.

In addition, in tests performed in the pulmonary laboratory nasal nitric oxide levels may be low.

Samples obtained by nasal brush biopsy can be analyzed and the ciliary movement recorded by video microscopy. Ciliary beat frequency (CBF) can be calculated in freshly isolated samples but also after growing the tissue in cell culture.

Furthermore, ciliated cells can be analyzed by electron microscopy to detect defects in the microscopic structure of the cilia.

The major problem encountered by all these assays is that they are performed under in vitro conditions and not in the natural environment of the cells where the acidity (pH), temperature, electrolytes etc. may be very different.

Moreover, genetic testing is limited to mutations known to cause PCD, ruling out mutations not yet discovered.

Therefore a method is needed to unequivocally measure ciliary movement in the natural environment.

Goal

  1. Assessment of a high speed video camera with a green light source for the meas
    Sponsor: Hadassah Medical Organization

    Current Primary Outcome:

    • Ciliary beat frequency (CBF) measured by high speed video microscopy [ Time Frame: 5 minutes ]
      Ex vivo measurements of CBF analysing high speed video records of nasal brush biopsies
    • Ciliary beat frequency (CBF) measured by interferometry [ Time Frame: 5 minutes ]
      In vivo measurements of CBF analysing the reflected light from the nasal cavity


    Original Primary Outcome: Same as current

    Current Secondary Outcome:

    Original Secondary Outcome:

    Information By: Hadassah Medical Organization

    Dates:
    Date Received: December 29, 2015
    Date Started: April 2016
    Date Completion: July 2019
    Last Updated: February 29, 2016
    Last Verified: February 2016