Clinical Trial: The Response of Pathogens to the Respective or Combined Treatment of SRP and Local Minocycline in Chronic Periodontitis

Study Status: Completed
Recruit Status: Completed
Study Type: Interventional

Official Title: The Response of Periodontal Pathogens to the Respective or Combined Treatment of Scaling and Root Planning and Locally Delivered Minocycline in Patients With Chronic Periodontiti

Brief Summary: To evaluate the respective or combinatory efficacy of locally delivered 2% Minocycline (MO) and surface and root planning (SRP) by assessing both clinical parameters and the loads of four main periodontal pathogens in treating chronic periodontitis.

Detailed Summary: In this randomized clinical trial, the investigators will evaluate the respective or combinatory efficacy of minocycline and scaling and root planning in the aspects of both clinical parameters [Pocket depth (PD) and sulcus bleeding index (SBI)] and the loads of four main periodontal pathogens [Aggregatibacter actinomycetemcomitans (Aa), Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg) and Prevotella intermedia (Pi)]. Real-time quantitative PCR (qRT-PCR) will be used as a powerful tool with high sensitivity and specificity to quantitatively assess target periodontal bacteria in a period of 7 days. The investigators will also try to correlate the reduction of either total or respective bacteria with the improvements of clinical parameters, with an aim to uncovering the potential microbiological mechanism accounting for the efficacy of a therapy.
Sponsor: Zhejiang University

Current Primary Outcome: Pocket Depth [ Time Frame: 7 days ]

PD is measured using a standard CPI（community periodontal index） probe (Shanghai Medical Instruments, Shanghai, China) and assessed to the nearest millimeter.


Original Primary Outcome: Same as current

Current Secondary Outcome:

  • Bleeding on Probing [ Time Frame: 7 days ]
    BOP is evaluated for the treated tooth using the sulcus bleeding index (SBI) by Muhlemann with a range of 0 (no bleeding) to 5 (profuse bleeding)
  • Bacterial Load [ Time Frame: 7 days ]
    Real-time quantitative PCR (qRT-PCR) was used as a powerful tool with high sensitivity and specificity to quantitatively assess target periodontal bacteria.


Original Secondary Outcome:

  • Bleeding on Probing [ Time Frame: 7 days ]
    BOP is evaluated for the treated tooth using the sulcus bleeding index (SBI) by Muhlemann with a range of 0 (no bleeding) to 5 (profuse bleeding)
  • Bacterial Load [ Time Frame: 7 days ]
    Real-time quantitative PCR (qRT-PCR)15, 16 was used as a powerful tool with high sensitivity and specificity to quantitatively assess target periodontal bacteria.


Information By: Zhejiang University

Dates:
Date Received: January 31, 2015
Date Started: June 2013
Date Completion:
Last Updated: October 29, 2015
Last Verified: October 2015