Clinical Trial: Collecting and Storing Tissue From Young Patients With Cancer

Study Status: Recruiting
Recruit Status: Recruiting
Study Type: Observational

Official Title: Establishing Continuous Cell Lines and Xenografts From Pediatric Cancers for Biological and Pre-Clinical Therapeutic Studies

Brief Summary:

RATIONALE: Collecting and storing samples of tissue, blood, and bone marrow from patients with cancer to study in the laboratory may help doctors learn more about changes that may occur in DNA and identify biomarkers related to cancer.

PURPOSE: This laboratory study is collecting and storing tissue, blood, and bone marrow samples from young patients with cancer.


Detailed Summary:

OBJECTIVES:

  • Establish and bank cell lines and/or xenografts from pediatric patients with cancer.
  • Establish continuous cell lines, under carefully controlled conditions, from pediatric patients with cancer.
  • Establish transplantable xenografts in immunocompromised mice from tumor cells that are difficult to establish as continuous cell lines in vitro.
  • Create a bank of cell lines and generate sufficient vials of cryopreserved cells for distribution to investigators with approved COG biology protocols.
  • Characterize cell lines from childhood cancers with respect to DNA short tandem repeat molecular profile as a "fingerprint" of original cell line identity.
  • Characterize cell lines for the ability for sustained growth in tissue culture and/or as mouse xenografts.
  • Characterize cell lines for mycoplasma contamination.
  • Characterize cell lines for expression of molecular makers that confirm the tumor-type of the cell line and the immortal nature of the cells (telomerase) and the expression of molecular markers that may correlate with drug resistance.

OUTLINE: This is a multicenter study. Specimens are stratified according to disease (acute lymphoblastic leukemia vs acute myeloid leukemia vs lymphoma vs osteogenic sarcoma vs Ewing family of tumors vs rhabdomyosarcoma vs primitive neuroectodermal tumor vs glioma vs astrocytoma vs rhabdoid tumors vs hepatoblastoma vs retinoblastoma vs Wilms tumor vs germ cell tumors vs other diagnoses).

Leftover tissue from diagnostic procedures and/or surgery is cr
Sponsor: Children's Oncology Group

Current Primary Outcome:

  • Establishment and banking of cell lines and/or xenografts from pediatric patients with cancer [ Time Frame: length of study ]
  • Establishment of continuous cell lines, under carefully controlled conditions, from pediatric patients with cancer [ Time Frame: length of study ]
  • Establishment of transplantable xenografts in immunocompromised mice from tumor cells that are difficult to establish as continuous cell lines in vitro [ Time Frame: length of study ]
  • Creation of a bank of cell lines and generation of sufficient vials of cryopreserved cells for distribution to investigators with approved COG biology protocols [ Time Frame: length of study ]
  • Characterization of cell lines from childhood cancers with respect to DNA PCR molecular HLA profile as a "fingerprint" of original cell line identity [ Time Frame: length of study ]
  • Characterization of cell lines for the ability for sustained growth in tissue culture and/or as mouse xenografts [ Time Frame: length of study ]
  • Characterization of cell lines for mycoplasma contamination [ Time Frame: length of study ]
  • Characterization of cell lines for expression of molecular makers that confirm the tumor-type of the cell line and the immortal nature of the cells (telomerase) and the expression of molecular markers that may correlate with drug resistance [ Time Frame: length of study ]


Original Primary Outcome:

  • Establishment and banking of cell lines and/or xenografts from pediatric patients with cancer
  • Establishment of continuous cell lines, under carefully controlled conditions, from pediatric patients with cancer
  • Establishment of transplantable xenografts in immunocompromised mice from tumor cells that are difficult to establish as continuous cell lines in vitro
  • Creation of a bank of cell lines and generation of sufficient vials of cryopreserved cells for distribution to investigators with approved COG biology protocols
  • Characterization of cell lines from childhood cancers with respect to DNA PCR molecular HLA profile as a "fingerprint" of original cell line identity
  • Characterization of cell lines for the ability for sustained growth in tissue culture and/or as mouse xenografts
  • Characterization of cell lines for mycoplasma contamination
  • Characterization of cell lines for expression of molecular makers that confirm the tumor-type of the cell line and the immortal nature of the cells (telomerase) and the expression of molecular markers that may correlate with drug resistance


Current Secondary Outcome:

Original Secondary Outcome:

Information By: Children's Oncology Group

Dates:
Date Received: May 9, 2009
Date Started: March 2007
Date Completion:
Last Updated: November 18, 2016
Last Verified: November 2016