Clinical Trial: Enteropathogenic Escherichia Coli (EPEC): Does it Have a Role in Colorectal Tumourigenesis?

Study Status: Completed
Recruit Status: Completed
Study Type: Observational [Patient Registry]

Official Title: ROLE OF E COLI IN Colorectal Tumourigenesis

Brief Summary: Despite the characterization of many aetiologic genetic changes. The specific causative factors in the development of sporadic colorectal cancer remain unclear. This study was performed to detect the possible role of Enteropathogenic Escherichia coli (EPEC) in developing colorectal carcinoma.

Detailed Summary: Fresh biopsy specimens have been obtained from the colonic mucosa overlying the colorectal cancer as well as from the colon of the healthy controls. Culture, genotyping and virulence of EPEC were done using (nutrient broth culture, and PCR). Strains biochemically identified as E. coli were selected from the surface of a MacConkey's plate and were serogrouped by slide agglutination tests
Sponsor: Mansoura University

Current Primary Outcome: virulence genes: eaeA, bfpA and stx. PCR [ Time Frame: 1 week ]

E. coli colonies isolated from the mucosa of colorectal cancers and from healthy controls were harvested and subjected to DNA extraction (QIAGEN)


Original Primary Outcome: Same as current

Current Secondary Outcome:

  • EPEC serogrouping: [ Time Frame: 1 week ]
    Strains biochemically identified as E. coli were selected from the surface of a MacConkey's plate and were serogrouped by slide agglutination tests to detect EPEC using O-specific nonavalent E.coli antiserum (O26, O55, O86, O111, O114, O119, O124, O125, O126, O127, O128, O142), according to the manufacturer's instructions (Bio-Rad).
  • Strains: OF E COLI [ Time Frame: 1 week ]
    The specimens were immediately transferred to the lab, inoculated in nutrient broth and vortexed for a few seconds then incubated overnight. The following day the broth culture was subcultured on enteric isolation media. Identification of E. coli was carried out by using standard biochemical methods.


Original Secondary Outcome: Same as current

Information By: Mansoura University

Dates:
Date Received: February 16, 2015
Date Started: January 2011
Date Completion:
Last Updated: April 7, 2015
Last Verified: February 2015