Clinical Trial: Molecular Biomarkers Associated With Degenerative Joint Disease in the Knee
Study Status: Completed
Recruit Status: Completed
Study Type: Observational
Official Title: Molecular Biomarkers Associated With Degenerative Joint Disease
Brief Summary: This investigation describes a novel complex of structural degradation proteins that is associated with pain in individuals with meniscal pathology undergoing arthroscopic partial meniscectomy. The presence of the complex in the synovial fluid of the knee is sensitive and specific for the painful state compared with similar aged controls. The absence of the complex in asymptomatic controls makes it a useful biomarker for the disease state.
Detailed Summary:
Background: Molecular biomarkers associated with knee pain may be useful as diagnostic tools, prognostic indicators, and surrogate endpoints for therapeutic trials. This study describes a novel complex of structural degradation proteins which are present in the knees of patients with meniscal pathology.
Methods: This prospective cohort study included 30 patients with knee pain, mechanical symptoms, and MRI positive for meniscus tear who elected for arthroscopic partial meniscectomy after failing conservative management. Synovial fluid was aspirated at the time of surgery and assayed for a complex of structural degradation proteins by means of heterogeneous ELISA. The results were compared to knee aspirates from 10 asymptomatic volunteers with no pain. The complex was identified by a proteomic analysis of including multiplexed-bead immunoassay, purification by anion exchange and size exclusion chromatography, and mass spectrometry of individual chromatographic fractions.
Sponsor: Scuderi, Gaetano J., M.D.
Current Primary Outcome:
Original Primary Outcome:
Current Secondary Outcome:
Original Secondary Outcome:
Information By: Scuderi, Gaetano J., M.D.
Dates:
Date Received: October 30, 2009
Date Started: January 2008
Date Completion:
Last Updated: October 30, 2009
Last Verified: October 2009
