Clinical Trial: Effects of Er,Cr:YSGG, Diode Lasers on GCF Cytokines Levels

Study Status: Completed
Recruit Status: Completed
Study Type: Interventional

Official Title: Comparison Of Er,Cr:YSGG and Diode Laser on the Periodontal Parameters and Cytokines Levels in Patients With Generalized Aggressive Periodontitis

Brief Summary: The aim of our study is to determine the interleukin-1βeta (IL-1β), interleukin-8 (IL-8) and tumor necrosis factor-αlfa (Tnf-α) levels in gingival crevicular fluid (GCF) and clinical periodontal parameters following the treatment with Erbium,Chromium:Yttrium-Scandium-Gallium-Garnet (Er,Cr:YSGG) and diode lasers in adjunct to scaling and root planing (SRP) in patients with generalized aggressive periodontitis (GAgP). Twenty-six patients with GAgP (n=26) were enrolled in the study. The study was designed as a "split-mouth" study. In each patient, three quadrants were randomly determined as SRP-control, SRP+Er,Cr:YSGG and SRP+Diode laser. Clinical periodontal measurements were recorded at the baseline and third month after the treatment. Cytokines levels in GCF were determined by enzyme-linked immunosorbent assay (ELISA).

Detailed Summary:

A total of 26 individuals were included in our study. Diagnoses were made after the clinical and radiographic examinations on patients who applied to the Yuzuncu Yil University Faculty of Dentistry, Department of Periodontology Clinics in 2014-2015. It was ensured that the individuals who were included in the study did not have any systemic diseases; were not menopausal, pregnant or lactating; had not used antibiotics or any other medication affecting the immune system in the previous six months; were non-smokers; had at least 16 teeth in their mouth; and had not received any periodontal treatment in the previous 6 months. The subjects involved in the study were told the aim and content of the study by a clinician, and they signed an approval form stating that they voluntarily participated in the survey. Each individual read the Helsinki Declaration before joining the study. The Human Ethics Research Committee of Yuzuncu Yil University (no.B.30.2.YYU.0.01.00.00/44-100912) gave the approval to carry out the study.

The including criteria were applied in diagnosing generalized aggressive periodontitis (International Workshop for Classification of Periodontal Diseases in 1999). Clinical periodontal assessments, including periodontal status, were conducted by measuring the bleeding on probing (BOP), plaque index (PI), gingival index (GI), probing pocket depth (PPD) and clinical attachment level (CAL) at 6 sites per tooth by another clinician. The clinical periodontal indices measured during the research were evaluated site-specifically by including the mesial and distal of those teeth from which GCF samples were obtained.

All individuals received non-surgical initial periodontal treatment. At the baseline of the study all quadrants were shown the similarity of periodontal disease. The procedures outlined below were applied to a
Sponsor: Yuzuncu Yıl University

Current Primary Outcome:

  • Periodontal Clinical Indices [ Time Frame: 1 year ]
    Bleeding on probing
  • Periodontal Clinical Indices [ Time Frame: 1 year ]
    Plaque index
  • Periodontal Clinical Indices [ Time Frame: 1 year ]
    Gingival index
  • Periodontal Clinical Indices [ Time Frame: 1 year ]
    Probing pocket depth
  • Periodontal Clinical Indices [ Time Frame: 1 year ]
    Clinical attachment level


Original Primary Outcome: Same as current

Current Secondary Outcome:

  • Cytokines levels in gingival crevicular fluids [ Time Frame: 1 year ]
    Interleukin-1β
  • Cytokines levels in gingival crevicular fluids [ Time Frame: 1 year ]
    Interleukin-8
  • Cytokines levels in gingival crevicular fluids [ Time Frame: 1 year ]
    Tumor necrosis factor-α


Original Secondary Outcome: Same as current

Information By: Yuzuncu Yıl University

Dates:
Date Received: December 21, 2016
Date Started: January 2014
Date Completion:
Last Updated: February 22, 2017
Last Verified: February 2017